Conjugation

Part:BBa_K1510915:Design

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-09)


ketoisocaproate decarboxylase (THI3) Circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 922
    Illegal EcoRI site found at 1621
    Illegal EcoRI site found at 1807
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.



Source

HI3 gene from Saccharomyces cerevisiae,BBa_B0034 is from 2014 igem distribution and BBa_B0015 is from 2014 igem distribution.

References